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An integrated microfluidic device for large-scale in situ click chemistry screening.

ABSTRACT: An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

SUBMITTER: Wang Y

PROVIDER: S-EPMC2878189 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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An integrated microfluidic device for large-scale in situ click chemistry screening.

Wang Yanju Y Lin Wei-Yu WY Liu Kan K Lin Rachel J RJ Selke Matthias M Kolb Hartmuth C HC Zhang Nangang N Zhao Xing-Zhong XZ Phelps Michael E ME Shen Clifton K F CK Faull Kym F KF Tseng Hsian-Rong HR

Lab on a chip 20090617 16

An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis. ...[more]

PMID: 19636457

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