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3D-reconstruction of blood vessels by ultramicroscopy.


ABSTRACT: As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with microm resolution. Thus, it can be applied to anatomical studies of numerous biological and medical specimens. We reconstructed the three-dimensional architecture of tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology of filigree branches of the microvasculature was visualized. Since tumors require an extensive growth of blood vessels to survive, this novel approach may open up new vistas in neurobiology and histology, particularly in cancer research.

SUBMITTER: Jahrling N 

PROVIDER: S-EPMC2878751 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

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3D-reconstruction of blood vessels by ultramicroscopy.

Jährling Nina N   Becker Klaus K   Dodt Hans-Ulrich HU  

Organogenesis 20091001 4


As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with microm resolution. Thus, it can be applied to anatomical studies of numerous biological and medical specimens. We reconstructed the three-dimensional architecture of tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology of filigree branches of the microvasculature was visualized. Since tumors require an extensive growth of blood vessels t  ...[more]

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