Dataset Information

3D histopathology of human tumours by fast clearing and ultramicroscopy.

ABSTRACT: Here, we describe a novel approach that allows pathologists to three-dimensionally analyse malignant tissues, including the tumour-host tissue interface. Our visualization technique utilizes a combination of ultrafast chemical tissue clearing and light-sheet microscopy to obtain virtual slices and 3D reconstructions of up to multiple centimetre sized tumour resectates. For the clearing of tumours we propose a preparation technique comprising three steps: (a) Fixation and enhancement of tissue autofluorescence with formalin/5-sulfosalicylic acid. (b) Ultrafast active chemical dehydration with 2,2-dimethoxypropane and (c) refractive index matching with dibenzyl ether at up to 56 °C. After clearing, the tumour resectates are imaged. The images are computationally post-processed for contrast enhancement and artefact removal and then 3D reconstructed. Importantly, the sequence a-c is fully reversible, allowing the morphological correlation of one and the same histological structures, once visualized with our novel technique and once visualized by standard H&E- and IHC-staining. After reverting the clearing procedure followed by standard H&E processing, the hallmarks of ductal carcinoma in situ (DCIS) found in the cleared samples could be successfully correlated with the corresponding structures present in H&E and IHC staining. Since the imaging of several thousands of optical sections is a fast process, it is possible to analyse a larger part of the tumour than by mechanical slicing. As this also adds further information about the 3D structure of malignancies, we expect that our technology will become a valuable addition for histological diagnosis in clinical pathology.

SUBMITTER: Sabdyusheva Litschauer I

PROVIDER: S-EPMC7572501 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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3D histopathology of human tumours by fast clearing and ultramicroscopy.

Sabdyusheva Litschauer Inna I Becker Klaus K Saghafi Saiedeh S Ballke Simone S Bollwein Christine C Foroughipour Meraaj M Gaugeler Julia J Foroughipour Massih M Schavelová Viktória V László Viktória V Döme Balazs B Brostjan Christine C Weichert Wilko W Dodt Hans-Ulrich HU

Scientific reports 20201019 1

Here, we describe a novel approach that allows pathologists to three-dimensionally analyse malignant tissues, including the tumour-host tissue interface. Our visualization technique utilizes a combination of ultrafast chemical tissue clearing and light-sheet microscopy to obtain virtual slices and 3D reconstructions of up to multiple centimetre sized tumour resectates. For the clearing of tumours we propose a preparation technique comprising three steps: (a) Fixation and enhancement of tissue au ...[more]

PMID: 33077794

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Dataset Information

3D histopathology of human tumours by fast clearing and ultramicroscopy.

Publications

3D histopathology of human tumours by fast clearing and ultramicroscopy.

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