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Novel candidate cancer genes identified by a large-scale cross-species comparative oncogenomics approach.

ABSTRACT: Comparative genomic hybridization (CGH) can reveal important disease genes but the large regions identified could sometimes contain hundreds of genes. Here we combine high-resolution CGH analysis of 598 human cancer cell lines with insertion sites isolated from 1,005 mouse tumors induced with the murine leukemia virus (MuLV). This cross-species oncogenomic analysis revealed candidate tumor suppressor genes and oncogenes mutated in both human and mouse tumors, making them strong candidates for novel cancer genes. A significant number of these genes contained binding sites for the stem cell transcription factors Oct4 and Nanog. Notably, mice carrying tumors with insertions in or near stem cell module genes, which are thought to participate in cell self-renewal, died significantly faster than mice without these insertions. A comparison of the profile we identified to that induced with the Sleeping Beauty (SB) transposon system revealed significant differences in the profile of recurrently mutated genes. Collectively, this work provides a rich catalogue of new candidate cancer genes for functional analysis.

SUBMITTER: Mattison J

PROVIDER: S-EPMC2880710 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Novel candidate cancer genes identified by a large-scale cross-species comparative oncogenomics approach.

Mattison Jenny J Kool Jaap J Uren Anthony G AG de Ridder Jeroen J Wessels Lodewyk L Jonkers Jos J Bignell Graham R GR Butler Adam A Rust Alistair G AG Brosch Markus M Wilson Catherine H CH van der Weyden Louise L Largaespada David A DA Stratton Michael R MR Futreal P Andy PA van Lohuizen Maarten M Berns Anton A Collier Lara S LS Hubbard Tim T Adams David J DJ

Cancer research 20100126 3

Comparative genomic hybridization (CGH) can reveal important disease genes but the large regions identified could sometimes contain hundreds of genes. Here we combine high-resolution CGH analysis of 598 human cancer cell lines with insertion sites isolated from 1,005 mouse tumors induced with the murine leukemia virus (MuLV). This cross-species oncogenomic analysis revealed candidate tumor suppressor genes and oncogenes mutated in both human and mouse tumors, making them strong candidates for no ...[more]

PMID: 20103622

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Dataset Information

Novel candidate cancer genes identified by a large-scale cross-species comparative oncogenomics approach.

Publications

Novel candidate cancer genes identified by a large-scale cross-species comparative oncogenomics approach.

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