Ontology highlight
ABSTRACT:
SUBMITTER: Tomishima MJ
PROVIDER: S-EPMC2881625 | biostudies-literature | 2007 Jan
REPOSITORIES: biostudies-literature
Tomishima Mark J MJ Hadjantonakis Anna-Katerina AK Gong Shiaoching S Studer Lorenz L
Stem cells (Dayton, Ohio) 20060921 1
Transgenic green fluorescent protein (GFP) reporter embryonic stem (ES) cells are powerful tools for studying gene regulation and lineage choice during development. Here we present a rapid method for the generation of ES cells expressing GFP under the control of selected genes. Bacterial artificial chromosomes (BACs) from a previously constructed GFP transcriptional fusion library (Gene Expression Nervous System Atlas [GENSAT]) were modified for use in ES cells, and multiple BAC transgenic ES ce ...[more]