Ontology highlight
ABSTRACT:
SUBMITTER: Harikumar A
PROVIDER: S-EPMC5639459 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Harikumar Arigela A Edupuganti Raghu Ram RR Sorek Matan M Azad Gajendra Kumar GK Markoulaki Styliani S Sehnalová Petra P Legartová Soňa S Bártová Eva E Farkash-Amar Shlomit S Jaenisch Rudolf R Alon Uri U Meshorer Eran E
Stem cell reports 20170928 4
Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse ESCs, we generated a library of over 200 endogenously tagged fluorescent fusion proteins and present several proof-of-concept applications of this library. We show the utility of this library to track proteins in living cells; screen for pluripo ...[more]