Ontology highlight
ABSTRACT:
SUBMITTER: Zhang J
PROVIDER: S-EPMC2882511 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Zhang Juan J Mendoza Mary M Guiraldelli Michel F MF Barbu Emilia Alina EA Siraganian Reuben P RP
Journal of immunology (Baltimore, Md. : 1950) 20100507 12
Mast cells play pivotal roles in the initiation of the allergic response. To gain an understanding of the functions played by phosphatases in IgE-mediated mast cell activation, a small interfering RNA (siRNA) library that targets all mouse phosphatase genes was screened in a mouse mast cell line, MMC-1. Of 198 targets, 10 enhanced and 7 inhibited FcepsilonRI-induced degranulation. For seven of the strongest hits, four different siRNAs per target were tested, and at least two out of the four sing ...[more]