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Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures.

ABSTRACT: After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report reviews the state of the art for phase plates and what is innovated with them in biological electron microscopy. The extension of thin-film phase plates to the material-less type using electrostatic field or magnetic field is also addressed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12551-008-0006-z) contains supplementary material, which is available to authorized users.

SUBMITTER: Nagayama K

PROVIDER: S-EPMC2883085 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Phase-plate electron microscopy: a novel imaging tool to reveal close-to-life nano-structures.

Nagayama Kuniaki K Danev Radostin R

Biophysical reviews 20090117 1

After slow progress in the efforts to develop phase plates for electron microscopes, functional phase plate using thin carbon film has been reported recently. It permits collecting high-contrast images of close-to-life biological structures with cryo-fixation and without staining. This report reviews the state of the art for phase plates and what is innovated with them in biological electron microscopy. The extension of thin-film phase plates to the material-less type using electrostatic field o ...[more]

PMID: 20585379

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