Ontology highlight
ABSTRACT:
SUBMITTER: Rostami A
PROVIDER: S-EPMC2884312 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Rostami A A Rabbani M M Mir-Mohammad-Sadeghi M M
Journal of biomolecular techniques : JBT 20100701 2
Glycosylation of the mu-opioid receptor may play an important role on its function. Using nested PCR, N53Q mutation was prepared in the N-terminal region of the rat mu-opioid receptor cDNA and cloned into the pcDNA3 vector. The plasmids containing the wild-type and mutated receptor cDNA were transfected into the COS-7 cells. Intracellular cAMP was measured in the morphine-treated and untreated transfected cells using an ELISA kit. Plasmid expression was evaluated using X-gal staining. Intracellu ...[more]