Optimized design and data analysis of tag-based cytosine methylation assays.
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ABSTRACT:
SUBMITTER: Suzuki M
PROVIDER: S-EPMC2884539 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Publications
Optimized design and data analysis of tag-based cytosine methylation assays.
Suzuki Masako M Jing Qiang Q Lia Daniel D Pascual Marién M McLellan Andrew A Greally John M JM
Genome biology 20100401 4
Using the type III restriction-modification enzyme EcoP15I, we isolated sequences flanking sites digested by the methylation-sensitive HpaII enzyme or its methylation-insensitive MspI isoschizomer for massively parallel sequencing. A novel data transformation allows us to normalise HpaII by MspI counts, resulting in more accurate quantification of methylation at >1.8 million loci in the human genome. This HELP-tagging assay is not sensitive to sequence polymorphism or base composition and allows ...[more]
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