Microglia activation mediates fibrillar amyloid-? toxicity in the aged primate cortex.

ABSTRACT: The amyloid-? peptide (A?) plays a central role in the pathogenesis of Alzheimer's disease (AD). The fibrillar form of A? (fA?) exerts toxic effects on neurons through mechanisms not well understood. We have shown that the aged primate cortex is selectively vulnerable to fA? toxicity at low concentrations. In addition to neuronal loss, fA? induced massive activation of microglia in the aged rhesus cortex. We now demonstrate that inhibition of microglia activation abolishes fA? toxicity. Injection or pump delivery of macrophage/microglia inhibitory factor (MIF) significantly reduced activation of microglia and the volume of damage caused by fA?. Microglia isolated from aged rhesus cortex produced substantial reactive oxygen species when stimulated by fA?, which was inhibited by MIF in a dose-dependent manner. This is the first definitive in vivo demonstration that the fA?-induced microglia activation and inflammation mediate, at least in part, its toxic effects on neurons. Combined with our earlier observations, these findings suggest that aged primate microglia may display an exaggerated inflammatory response to fA? when compared with young microglia.

SUBMITTER: Leung E 

PROVIDER: S-EPMC2888944 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature