Ontology highlight
ABSTRACT:
SUBMITTER: Lee MK
PROVIDER: S-EPMC2889053 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Lee Mi-Kyung MK Gal Maayan M Frydman Lucio L Varani Gabriele G
Proceedings of the National Academy of Sciences of the United States of America 20100503 20
Conformational transitions and structural rearrangements are central to the function of many RNAs yet remain poorly understood. We have used ultrafast multidimensional NMR techniques to monitor the adenine-induced folding of an adenine-sensing riboswitch in real time, with nucleotide-resolved resolution. By following changes in 2D spectra at rates of approximately 0.5 Hz, we identify distinct steps associated with the ligand-induced folding of the riboswitch. Following recognition of the ligand, ...[more]