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Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos.

ABSTRACT: Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every gene of the mouse genome. To realize the utility of these resources, efficient and accessible methods of generating mutant mice from these ES cells are necessary. Here, we describe a combination of ICR morula aggregation and a chemically-defined culture medium with widely available and accessible components for the high efficiency generation of germline transmitting chimeras from C57BL/6N ES cells. Together these methods will ease the access of the broader biomedical research community to the publicly available B6 ES cell resources.

SUBMITTER: Gertsenstein M

PROVIDER: S-EPMC2889837 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos.

Gertsenstein Marina M Nutter Lauryl M J LM Reid Tammy T Pereira Monica M Stanford William L WL Rossant Janet J Nagy Andras A

PloS one 20100622 6

Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every gene of the mouse genome. To realize the utility of these resources, efficient and accessible methods of generating mutant mice from these ES cells are necessary. Here, we describe a combination of ICR morula aggreg ...[more]

PMID: 20582321

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