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Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy.

ABSTRACT: We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

SUBMITTER: Tonzetich ZJ

PROVIDER: S-EPMC2892860 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Identification of protein-bound dinitrosyl iron complexes by nuclear resonance vibrational spectroscopy.

Tonzetich Zachary J ZJ Wang Hongxin H Mitra Devrani D Tinberg Christine E CE Do Loi H LH Jenney Francis E FE Adams Michael W W MW Adams Michael W W MW Cramer Stephen P SP Lippard Stephen J SJ

Journal of the American Chemical Society 20100501 20

We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitri ...[more]

PMID: 20429508

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