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Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition.

ABSTRACT: We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

SUBMITTER: Han HS

PROVIDER: S-EPMC2892974 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition.

Han Hee-Sun HS Devaraj Neal K NK Lee Jungmin J Hilderbrand Scott A SA Weissleder Ralph R Bawendi Moungi G MG

Journal of the American Chemical Society 20100601 23

We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins. ...[more]

PMID: 20481508

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