Comparison of normalization methods for construction of large, multiplex amplicon pools for next-generation sequencing.
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SUBMITTER: Harris JK
PROVIDER: S-EPMC2893486 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
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Comparison of normalization methods for construction of large, multiplex amplicon pools for next-generation sequencing.
Harris J Kirk JK Sahl Jason W JW Castoe Todd A TA Wagner Brandie D BD Pollock David D DD Spear John R JR
Applied and environmental microbiology 20100423 12
Constructing mixtures of tagged or bar-coded DNAs for sequencing is an important requirement for the efficient use of next-generation sequencers in applications where limited sequence data are required per sample. There are many applications in which next-generation sequencing can be used effectively to sequence large mixed samples; an example is the characterization of microbial communities where <or=1,000 sequences per samples are adequate to address research questions. Thus, it is possible to ...[more]
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