Ontology highlight
ABSTRACT:
SUBMITTER: Sykes MT
PROVIDER: S-EPMC2894367 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Sykes Michael T MT Sperling Edit E Chen Stephen S SS Williamson James R JR
Analytical chemistry 20100601 12
Relative levels of ribosomal proteins were quantified in crude cell lysates using mass spectrometry. A method for quantifying cellular protein levels using macromolecular standards is presented that does not require complex sample separation, identification of high-responding peptides, affinity purification, or postgrowth modifications. Perturbations in ribosomal protein levels by overexpression of individual proteins correlate to known autoregulatory mechanisms and extend the network of ribosom ...[more]