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Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42.


ABSTRACT: BACKGROUND: Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP. RESULTS: Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, we found that only the proteasome subunit p42 from human fetal lung diploid fibroblast (2BS) cells bound to SARS-CoV NP. This interaction was confirmed by the glutathione S-transferase (GST) fusion protein pulldown technique. The co-localization signal of SARS-CoV NP and proteasome subunit p42 in 2BS cells was detected using indirect immunofluorescence and confocal microscopy. p42 is a subunit of the 26S proteasome; this large, multi-protein complex is a component of the ubiquitin-proteasome pathway, which is involved in a variety of basic cellular processes and inflammatory responses. CONCLUSION: To our knowledge, this is the first report that SARS-CoV NP interacts with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS-CoV pathogenicity and the means by which SARS-CoV interacts with host cells.

SUBMITTER: Wang Q 

PROVIDER: S-EPMC2894783 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

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Interactions of SARS coronavirus nucleocapsid protein with the host cell proteasome subunit p42.

Wang Qin Q   Li Chuan C   Zhang Quanfu Q   Wang Tao T   Li Jiandong J   Guan Wuxiang W   Yu Jianshi J   Liang Mifang M   Li Dexin D  

Virology journal 20100517


<h4>Background</h4>Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP.<h4>Results</h4>Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, we found that only  ...[more]

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