Ontology highlight
ABSTRACT:
SUBMITTER: Sachdeva R
PROVIDER: S-EPMC2895063 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Sachdeva Rohit R Jönsson Marie E ME Nelander Jenny J Kirkeby Agnete A Guibentif Carolina C Gentner Bernhard B Naldini Luigi L Björklund Anders A Parmar Malin M Jakobsson Johan J
Proceedings of the National Academy of Sciences of the United States of America 20100607 25
In this study, we have used a microRNA-regulated lentiviral reporter system to visualize and segregate differentiating neuronal cells in pluripotent cultures. Efficient suppression of transgene expression, specifically in undifferentiated pluripotent cells, was achieved by using a lentiviral vector expressing a fluorescent reporter gene regulated by microRNA-292. Using this strategy, it was possible to track progeny from murine ES, human ES cells, and induced pluripotent stem cells as they diffe ...[more]