Dataset Information

Detection of novel biomarkers of liver cirrhosis by proteomic analysis.

ABSTRACT: Hepatic cirrhosis is a life-threatening disease arising from different chronic liver disorders. One major cause for hepatic cirrhosis is chronic hepatitis C. Chronic hepatitis C is characterized by a highly variable clinical course, with at least 20% developing liver cirrhosis within 40 years. Only liver biopsy allows a reliable evaluation of the course of hepatitis C by grading inflammation and staging fibrosis, and thus serum biomarkers for hepatic fibrosis with high sensitivity and specificity are needed. To identify new candidate biomarkers for hepatic fibrosis, we performed a proteomic approach of microdissected cirrhotic septa and liver parenchyma cells. In cirrhotic septa, we detected an increasing expression of cell structure associated proteins, including actin, prolyl 4-hydroxylase, tropomyosin, calponin, transgelin, and human microfibril-associated protein 4 (MFAP-4). Tropomyosin, calponin, and transgelin reflect a contribution of activated stellate cells/myofibroblasts to chronic liver injury. The expression of tropomyosin, transgelin, and MFAP-4, an extracellular matrix associated protein, were further evaluated by immunohistochemistry. Tropomyosin and MFAP-4 demonstrated high serum levels in patients with hepatic cirrhosis of different causes.A quantitative analysis of MFAP-4 serum levels in a large number of patients showed MFAP-4 as novel candidate biomarker with high diagnostic accuracy for prediction of nondiseased liver versus cirrhosis [area under receiver operating characteristic curve (AUC) = 0.97, P < 0.0001] as well as stage 0 versus stage 4 fibrosis (AUC = 0.84, P < 0.0001), and stages 0 to 3 versus stage 4 fibrosis (AUC = 0.76, P < 0.0001).

SUBMITTER: Molleken C

PROVIDER: S-EPMC2895500 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Detection of novel biomarkers of liver cirrhosis by proteomic analysis.

Mölleken Christian C Sitek Barbara B Henkel Corinna C Poschmann Gereon G Sipos Bence B Wiese Sebastian S Warscheid Bettina B Broelsch Christoph C Reiser Markus M Friedman Scott L SL Tornøe Ida I Schlosser Anders A Klöppel Günter G Schmiegel Wolff W Meyer Helmut E HE Holmskov Uffe U Stühler Kai K

Hepatology (Baltimore, Md.) 20090401 4

<h4>Unlabelled</h4>Hepatic cirrhosis is a life-threatening disease arising from different chronic liver disorders. One major cause for hepatic cirrhosis is chronic hepatitis C. Chronic hepatitis C is characterized by a highly variable clinical course, with at least 20% developing liver cirrhosis within 40 years. Only liver biopsy allows a reliable evaluation of the course of hepatitis C by grading inflammation and staging fibrosis, and thus serum biomarkers for hepatic fibrosis with high sensiti ...[more]

PMID: 19177598

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Dataset Information

Detection of novel biomarkers of liver cirrhosis by proteomic analysis.

Publications

Detection of novel biomarkers of liver cirrhosis by proteomic analysis.

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