Unknown

Dataset Information

0

Generation of functional ventricular heart muscle from mouse ventricular progenitor cells.


ABSTRACT: The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic stem cells. Genome-wide profiling of coding and noncoding transcripts revealed distinct molecular signatures of these progenitor populations. We further identify a committed ventricular progenitor cell in the Islet 1 lineage that is capable of limited in vitro expansion, differentiation, and assembly into functional ventricular muscle tissue, representing a combination of tissue engineering and stem cell biology.

SUBMITTER: Domian IJ 

PROVIDER: S-EPMC2895998 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Generation of functional ventricular heart muscle from mouse ventricular progenitor cells.

Domian Ibrahim J IJ   Chiravuri Murali M   van der Meer Peter P   Feinberg Adam W AW   Shi Xi X   Shao Ying Y   Wu Sean M SM   Parker Kevin Kit KK   Chien Kenneth R KR  

Science (New York, N.Y.) 20091001 5951


The mammalian heart is formed from distinct sets of first and second heart field (FHF and SHF, respectively) progenitors. Although multipotent progenitors have previously been shown to give rise to cardiomyocytes, smooth muscle, and endothelial cells, the mechanism governing the generation of large numbers of differentiated progeny remains poorly understood. We have employed a two-colored fluorescent reporter system to isolate FHF and SHF progenitors from developing mouse embryos and embryonic s  ...[more]

Similar Datasets

| S-EPMC3902294 | biostudies-literature
| S-EPMC6561316 | biostudies-literature
| S-EPMC7291744 | biostudies-literature
| S-EPMC373505 | biostudies-literature
| S-EPMC3400969 | biostudies-literature
| S-EPMC1635164 | biostudies-literature
| S-EPMC7409690 | biostudies-literature
| S-EPMC4675512 | biostudies-literature
| S-EPMC6590775 | biostudies-literature
| S-EPMC3973076 | biostudies-literature