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Practical method for extraction of PCR-quality DNA from environmental soil samples.

ABSTRACT: Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.

SUBMITTER: Fitzpatrick KA 

PROVIDER: S-EPMC2897455 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Practical method for extraction of PCR-quality DNA from environmental soil samples.

Fitzpatrick Kelly A KA   Kersh Gilbert J GJ   Massung Robert F RF  

Applied and environmental microbiology 20100430 13

Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples. ...[more]

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