Practical method for extraction of PCR-quality DNA from environmental soil samples.
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ABSTRACT:
SUBMITTER: Fitzpatrick KA
PROVIDER: S-EPMC2897455 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Publications
Practical method for extraction of PCR-quality DNA from environmental soil samples.
Fitzpatrick Kelly A KA Kersh Gilbert J GJ Massung Robert F RF
Applied and environmental microbiology 20100430 13
Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples. ...[more]
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