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Nanoscale imaging of molecular positions and anisotropies.

ABSTRACT: Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibroblasts expressing Dendra2-actin or Dendra2-hemagglutinin.

SUBMITTER: Gould TJ

PROVIDER: S-EPMC2901392 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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Nanoscale imaging of molecular positions and anisotropies.

Gould Travis J TJ Gunewardene Mudalige S MS Gudheti Manasa V MV Verkhusha Vladislav V VV Yin Shu-Rong SR Gosse Julie A JA Hess Samuel T ST

Nature methods 20081116 12

Knowledge of the orientation of molecules within biological structures is crucial to understanding the mechanisms of cell function. We present a method to image simultaneously the positions and fluorescence anisotropies of large numbers of single molecules with nanometer lateral resolution within a sample. Based on a simple modification of fluorescence photoactivation localization microscopy (FPALM), polarization (P)-FPALM does not compromise speed or sensitivity. We show results for mouse fibro ...[more]

PMID: 19011626

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