Ontology highlight
ABSTRACT:
SUBMITTER: Santoro SW
PROVIDER: S-EPMC290271 | biostudies-literature | 2003 Dec
REPOSITORIES: biostudies-literature
Santoro Stephen W SW Anderson J Christopher JC Lakshman Vishva V Schultz Peter G PG
Nucleic acids research 20031201 23
The addition of novel amino acids to the genetic code of Escherichia coli involves the generation of an aminoacyl-tRNA synthetase and tRNA pair that is 'orthogonal', meaning that it functions independently of the synthetases and tRNAs endogenous to E.coli. The amino acid specificity of the orthogonal synthetase is then modified to charge the corresponding orthogonal tRNA with an unnatural amino acid that is subsequently incorporated into a polypeptide in response to a nonsense or missense codon. ...[more]