Ontology highlight
ABSTRACT:
SUBMITTER: Nautiyal S
PROVIDER: S-EPMC2906552 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Nautiyal Shivani S Carlton Victoria E H VE Lu Yontao Y Ireland James S JS Flaucher Diane D Moorhead Martin M Gray Joe W JW Spellman Paul P Mindrinos Michael M Berg Paul P Faham Malek M
Proceedings of the National Academy of Sciences of the United States of America 20100623 28
A unique microarray-based method for determining the extent of DNA methylation has been developed. It relies on a selective enrichment of the regions to be assayed by target amplification by capture and ligation (mTACL). The assay is quantitatively accurate, relatively precise, and lends itself to high-throughput determination using nanogram amounts of DNA. The measurements using mTACLs are highly reproducible and in excellent agreement with those obtained by sequencing (r = 0.94). In the presen ...[more]