Ontology highlight
ABSTRACT:
SUBMITTER: Morrill BH
PROVIDER: S-EPMC3934139 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Morrill Benson H BH Cox Lindsay L Ward Anika A Heywood Sierra S Prather Randall S RS Isom S Clay SC
The Journal of reproduction and development 20130222 3
The purpose of this experiment was to implement and evaluate the effectiveness of a next-generation sequencing-based method for DNA methylation analysis in porcine embryonic samples. Fourteen discrete genomic regions were amplified by PCR using bisulfite-converted genomic DNA derived from day 14 in vivo-derived (IVV) and parthenogenetic (PA) porcine embryos as template DNA. Resulting PCR products were subjected to high-throughput sequencing using the Illumina Genome Analyzer IIx platform. The av ...[more]