Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies.
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ABSTRACT:
SUBMITTER: Brattelid T
PROVIDER: S-EPMC2907514 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Publications
Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies.
Brattelid Trond T Winer Lisbeth H LH Levy Finn Olav FO Liestøl Knut K Sejersted Ole M OM Andersson Kristin B KB
BMC molecular biology 20100323
<h4>Background</h4>Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for mRNA quantification, but requires invariant expression of the chosen reference gene(s). In pathological myocardium, there is limited information on suitable reference genes other than the commonly used Gapdh mRNA and 18S ribosomal RNA. Our aim was to evaluate and identify suitable reference genes in human failing myocardium, in rat and mouse post-myocardial infarction (post-MI) heart failure and across de ...[more]
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