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Catalysis of the microtubule on-rate is the major parameter regulating the depolymerase activity of MCAK.

ABSTRACT: The kinesin-13, MCAK, is a critical regulator of microtubule dynamics in eukaryotic cells. We have functionally dissected the structural features responsible for MCAK's potent microtubule depolymerization activity. MCAK's positively charged neck enhances its delivery to microtubule ends not by tethering the molecule to microtubules during diffusion, as commonly thought, but by catalyzing the association of MCAK to microtubules. On the other hand, this same positively charged neck slightly diminishes MCAK's ability to remove tubulin subunits once at the microtubule end. Conversely, dimerization reduces MCAK delivery but improves MCAK's ability to remove tubulin subunits. The reported kinetics for these events predicts a nonspecific binding mechanism that may represent a paradigm for the diffusive interaction of many microtubule-binding proteins.

SUBMITTER: Cooper JR

PROVIDER: S-EPMC2909650 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Catalysis of the microtubule on-rate is the major parameter regulating the depolymerase activity of MCAK.

Cooper Jeremy R JR Wagenbach Michael M Asbury Charles L CL Wordeman Linda L

Nature structural & molecular biology 20091206 1

The kinesin-13, MCAK, is a critical regulator of microtubule dynamics in eukaryotic cells. We have functionally dissected the structural features responsible for MCAK's potent microtubule depolymerization activity. MCAK's positively charged neck enhances its delivery to microtubule ends not by tethering the molecule to microtubules during diffusion, as commonly thought, but by catalyzing the association of MCAK to microtubules. On the other hand, this same positively charged neck slightly dimini ...[more]

PMID: 19966798

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