Ontology highlight
ABSTRACT:
SUBMITTER: Fili N
PROVIDER: S-EPMC2910053 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Fili Natali N Mashanov Gregory I GI Toseland Christopher P CP Batters Christopher C Wallace Mark I MI Yeeles Joseph T P JT Dillingham Mark S MS Webb Martin R MR Molloy Justin E JE
Nucleic acids research 20100328 13
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes move processively along DNA. Here, an optical method has been developed to follow the unwinding of multiple DNA molecules simultaneously in real time. This was achieved by measuring the accumulation of fluorescent single-stranded DNA-binding prot ...[more]