Ontology highlight
ABSTRACT:
SUBMITTER: Busch CJ
PROVIDER: S-EPMC2912859 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Busch Clara Jana-Lui CJ Vogt Alexander A Mochizuki Kazufumi K
BMC microbiology 20100713
<h4>Background</h4>Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding.<h4>Results</h4>In this study, we have established a Cre/loxP recombination system in Tetrahymena and have applied this system for the N-terminal epitope tagging of Tetrahymena genes. Cre can be expressed in Tetrahy ...[more]