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Modules for C-terminal epitope tagging of Tetrahymena genes.

ABSTRACT: Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.

SUBMITTER: Kataoka K

PROVIDER: S-EPMC2935961 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Modules for C-terminal epitope tagging of Tetrahymena genes.

Kataoka Kensuke K Schoeberl Ursula E UE Mochizuki Kazufumi K

Journal of microbiological methods 20100717 3

Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies. ...[more]

PMID: 20624430

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