Quantifying a pathway: kinetic analysis of actin dendritic nucleation.

ABSTRACT: Progress in uncovering the reaction networks that underlie important cell functions is laying the groundwork for quantitative identification of protein-interaction pathways. Since direct measurement of rate constants is not always feasible, the parameters are often inferred from multiple pieces of data using kinetic analyses based on appropriate mathematical models. The success of this approach relies on the sufficiency of available experimental data for a unique parameterization of the network. The concept of a rate-limiting step is applied to the analysis of experimental data that are usually used to quantify a pathway of actin dendritic nucleation, the Arp2/3-mediated mechanism that enables rapid changes of cell shape in response to external cues. The method yields analytical descriptio

SUBMITTER: Kraikivski P 

PROVIDER: S-EPMC2913184 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature