Ontology highlight
ABSTRACT:
SUBMITTER: Miyoshi T
PROVIDER: S-EPMC2064704 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Miyoshi Takushi T Tsuji Takahiro T Higashida Chiharu C Hertzog Maud M Fujita Akiko A Narumiya Shuh S Scita Giorgio G Watanabe Naoki N
The Journal of cell biology 20061201 6
Actin forms the dendritic nucleation network and undergoes rapid polymerization-depolymerization cycles in lamellipodia. To elucidate the mechanism of actin disassembly, we characterized molecular kinetics of the major filament end-binding proteins Arp2/3 complex and capping protein (CP) using single-molecule speckle microscopy. We have determined the dissociation rates of Arp2/3 and CP as 0.048 and 0.58 s(-1), respectively, in lamellipodia of live XTC fibroblasts. This CP dissociation rate is t ...[more]