Ontology highlight
ABSTRACT:
SUBMITTER: Dilworth DJ
PROVIDER: S-EPMC2914207 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Dilworth David J DJ Saleem Ramsey A RA Rogers Richard S RS Mirzaei Hamid H Boyle John J Aitchison John D JD
Journal of the American Society for Mass Spectrometry 20100408 8
Stable incorporation of labeled amino acids in cell culture is a simple approach to label proteins in vivo for mass spectrometric quantification. Full incorporation of isotopically heavy amino acids facilitates accurate quantification of proteins from different cultures, yet analysis methods for determination of incorporation are cumbersome and time-consuming. We present QTIPS, Quantification by Total Identified Peptides for SILAC, a straightforward, accurate method to determine the level of hea ...[more]