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Performance evaluation of three liquid chromatography mass spectrometry methods for broad spectrum drug screening.

ABSTRACT: Liquid chromatography-mass spectrometry (LC-MS) and tandem LC-MS (LC-MS/MS) are increasingly used in toxicology laboratories as a complementary method to gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-ultraviolet detection (LC-UV) for comprehensive drug screening (CDS). This study was designed to characterize the sensitivity and specificity of three LC-MS(/MS) vendor-supplied methods for targeted CDS and identify the current limitations associated with the use of these technologies.Five methods for broad spectrum CDS, including LC-UV (REMEDi), full scan GC-MS, LC-MS (ZQ-Mass Detector with MassLynx-software), LC-QTRAP-MS/MS (3200-QTRAP with Cliquid-software) and LC-LIT-MS/MS (LXQ Linear Ion Trap with ToxID-software) were evaluated based on their ability to detect drugs in 48 patient urine samples.The tandem MS methods identified 15% more drugs than the single stage MS or LC-UV methods. Use of two broad spectrum screening methods identified more drugs than any single system alone. False negatives and false positives generated by the LC-MS(/MS) software programs were identified upon manual review of the raw data.The LC-MS/MS methods detected a broader menu of drugs; however, it is essential to establish manual data review criteria for all LC-MS(/MS) drug screening methods. Use of an EI-GC-MS and ESI-LC-MS/MS combination for targeted CDS may be optimal due to the complementary nature of the chromatographic and ionization techniques.

SUBMITTER: Lynch KL

PROVIDER: S-EPMC2914548 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Performance evaluation of three liquid chromatography mass spectrometry methods for broad spectrum drug screening.

Lynch Kara L KL Breaud Autumn R AR Vandenberghe Hilde H Wu Alan H B AH Clarke William W

Clinica chimica acta; international journal of clinical chemistry 20100609 19-20

<h4>Background</h4>Liquid chromatography-mass spectrometry (LC-MS) and tandem LC-MS (LC-MS/MS) are increasingly used in toxicology laboratories as a complementary method to gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-ultraviolet detection (LC-UV) for comprehensive drug screening (CDS). This study was designed to characterize the sensitivity and specificity of three LC-MS(/MS) vendor-supplied methods for targeted CDS and identify the current limitations associated with ...[more]

PMID: 20540936

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Dataset Information

Performance evaluation of three liquid chromatography mass spectrometry methods for broad spectrum drug screening.

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Performance evaluation of three liquid chromatography mass spectrometry methods for broad spectrum drug screening.

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