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High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6')-Ib-cr.

ABSTRACT: We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6')-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6')-Ib and aac(6')-Ib-cr coexist.

SUBMITTER: Hidalgo-Grass C

PROVIDER: S-EPMC2916293 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6')-Ib-cr.

Hidalgo-Grass Carlos C Strahilevitz Jacob J

Antimicrobial agents and chemotherapy 20100524 8

We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6')-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6')-Ib and aac(6')-Ib-cr coexist. ...[more]

PMID: 20498320

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