Ontology highlight
ABSTRACT:
SUBMITTER: Menzella HG
PROVIDER: S-EPMC2916480 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Menzella Hugo G HG Carney John R JR Li Yong Y Santi Daniel V DV
Applied and environmental microbiology 20100611 15
Recombinant microbial whole-cell biocatalysis is a valuable approach for producing enantiomerically pure intermediates for the synthesis of complex molecules. Here, we describe a method to produce polyketide intermediates possessing multiple stereogenic centers by combining chemobiosynthesis and engineered mini-polyketide synthases (PKSs). Chemobiosynthesis allows the introduction of unnatural moieties, while a library of synthetic bimodular PKSs expressed from codon-optimized genes permits the ...[more]