Dataset Information

Localized fetomaternal hyperglycemia: spatial and kinetic definition by positron emission tomography.

ABSTRACT:

Background

Complex but common maternal diseases such as diabetes and obesity contribute to adverse fetal outcomes. Understanding of the mechanisms involved is hampered by difficulty in isolating individual elements of complex maternal states in vivo. We approached this problem in the context of maternal diabetes and sought an approach to expose the developing fetus in vivo to isolated hyperglycemia in the pregnant rat.

Methodology and principal findings

We hypothesized that glucose infused into the arterial supply of one uterine horn would more highly expose fetuses in the ipsilateral versus contralateral uterine horn. To test this, the glucose tracer [18F]fluorodeoxyglucose (FDG) was infused via the left uterine artery. Regional glucose uptake into maternal tissues and fetuses was quantified using positron emission tomography (PET). Upon infusion, FDG accumulation began in the left-sided placentae, subsequently spreading to the fetuses. Over two hours after completion of the infusion, FDG accumulation was significantly greater in left compared to right uterine horn fetuses, favoring the left by 1.9+/-0.1 and 2.8+/-0.3 fold under fasted and hyperinsulinemic conditions (p<10(-11) n=32-35 and p<10(-12) n=27-45) respectively. By contrast, centrally administered [3H]-2-deoxyglucose accumulated equally between the fetuses of the two uterine horns. Induction of significant hyperglycemia (10(3) mg/dL) localized to the left uterine artery was sustained for at least 48 hours while maternal euglycemia was maintained.

Conclusions and significance

This approach exposes selected fetuses to localized hyperglycemia in vivo, minimizing exposure of the mother and thus secondary effects. Additionally, a set of less exposed internal control fetuses are maintained for comparison, allowing direct study of the in vivo fetal effects of isolated hyperglycemia. Broadly, this approach can be extended to study a variety of maternal-sided perturbations suspected to directly affect fetal health.

SUBMITTER: Yao J

PROVIDER: S-EPMC2917372 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Publications

Localized fetomaternal hyperglycemia: spatial and kinetic definition by positron emission tomography.

Yao Jianrong J Wang Chunlin C Walsh Susan A SA Hu Shanming S Sawatzke Alexander B AB Dang Diana D Segar Jeffrey L JL Ponto Laura L B LL Sunderland John J JJ Norris Andrew W AW

PloS one 20100806 8

<h4>Background</h4>Complex but common maternal diseases such as diabetes and obesity contribute to adverse fetal outcomes. Understanding of the mechanisms involved is hampered by difficulty in isolating individual elements of complex maternal states in vivo. We approached this problem in the context of maternal diabetes and sought an approach to expose the developing fetus in vivo to isolated hyperglycemia in the pregnant rat.<h4>Methodology and principal findings</h4>We hypothesized that glucos ...[more]

PMID: 20700464

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Dataset Information

Localized fetomaternal hyperglycemia: spatial and kinetic definition by positron emission tomography.

Background

Methodology and principal findings

Conclusions and significance

Publications

Localized fetomaternal hyperglycemia: spatial and kinetic definition by positron emission tomography.

Similar Datasets

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