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Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.

ABSTRACT: GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.

SUBMITTER: Jinek M

PROVIDER: S-EPMC2920127 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.

Jinek Martin M Fabian Marc R MR Coyle Scott M SM Sonenberg Nahum N Doudna Jennifer A JA

Nature structural & molecular biology 20100124 2

GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing. ...[more]

PMID: 20098421

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