Ontology highlight
ABSTRACT:
SUBMITTER: Raisch T
PROVIDER: S-EPMC6639331 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Nature communications 20190718 1
CCR4-NOT is a conserved multiprotein complex which regulates eukaryotic gene expression principally via shortening of poly(A) tails of messenger RNA or deadenylation. Here, we reconstitute a complete, recombinant human CCR4-NOT complex. Our reconstitution strategy permits strict compositional control to test mechanistic hypotheses with purified component variants. CCR4-NOT is more active and selective for poly(A) than the isolated exonucleases, CCR4a and CAF1, which have distinct deadenylation p ...[more]