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Detecting and understanding the roles of nitric oxide in biology.

ABSTRACT: We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron-sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron-sulfur proteins. Our studies reveal that NO disassembles the Fe-S clusters to form dinitrosyl iron complexes.

SUBMITTER: Tonzetich ZJ

PROVIDER: S-EPMC2922020 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Detecting and understanding the roles of nitric oxide in biology.

Tonzetich Zachary J ZJ McQuade Lindsey E LE Lippard Stephen J SJ

Inorganic chemistry 20100701 14

We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to e ...[more]

PMID: 20666391

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