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ABSTRACT:
SUBMITTER: Wakabayashi S
PROVIDER: S-EPMC2924106 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Wakabayashi Shigeo S Nakamura Tomoe Y TY Kobayashi Soushi S Hisamitsu Takashi T
The Journal of biological chemistry 20100615 34
Protein kinase C (PKC) is considered crucial for hormonal Na(+)/H(+) exchanger (NHE1) activation because phorbol esters (PEs) strongly activate NHE1. However, here we report that rather than PKC, direct binding of PEs/diacylglycerol to the NHE1 lipid-interacting domain (LID) and the subsequent tighter association of LID with the plasma membrane mainly underlies NHE1 activation. We show that (i) PEs directly interact with the LID of NHE1 in vitro, (ii) like PKC, green fluorescent protein (GFP)-la ...[more]