Ontology highlight
ABSTRACT:
SUBMITTER: Liu G
PROVIDER: S-EPMC2924473 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Liu Guoqi G Chen Xiaomi X Bissler John J JJ Sinden Richard R RR Leffak Michael M
Nature chemical biology 20100801 9
Instability of (CTG) x (CAG) microsatellite trinucleotide repeat (TNR) sequences is responsible for more than a dozen neurological or neuromuscular diseases. TNR instability during DNA synthesis is thought to involve slipped-strand or hairpin structures in template or nascent DNA strands, although direct evidence for hairpin formation in human cells is lacking. We have used targeted recombination to create a series of isogenic HeLa cell lines in which (CTG) x (CAG) repeats are replicated from an ...[more]