Oligonucleotide frequencies of barcoding loci can discriminate species across kingdoms.

ABSTRACT:

Background

DNA barcoding refers to the use of short DNA sequences for rapid identification of species. Genetic distance or character attributes of a particular barcode locus discriminate the species. We report an efficient approach to analyze short sequence data for discrimination between species.

Methodology and principal findings

A new approach, Oligonucleotide Frequency Range (OFR) of barcode loci for species discrimination is proposed. OFR of the loci that discriminates between species was characteristic of a species, i.e., the maxima and minima within a species did not overlap with that of other species. We compared the species resolution ability of different barcode loci using p-distance, Euclidean distance of oligonucleotide frequencies, nucleotide-character based app

SUBMITTER: Tyagi A 

PROVIDER: S-EPMC2924895 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature