Ontology highlight
ABSTRACT:
SUBMITTER: Christov PP
PROVIDER: S-EPMC2925382 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Journal of nucleic acids 20100725
Formamidopyrimidines (Fapy) lesions result from ring opening of the imidazole portion of purines. Fapy lesions can isomerize from the natural beta-anomeric stereochemistry to the alpha-configuration. We have unambiguously demonstrated that the alpha-methyl-Fapy-dG (MeFapy-dG) lesion is a substrate for Escherichia coli Endonuclease IV (Endo IV). Treatment of a MeFapy-dG-containing 24 mer duplex with Endo IV resulted in 36-40% incision. The catalytic efficiency of the incision was comparable to th ...[more]