Ontology highlight
ABSTRACT:
SUBMITTER: Popoff SC
PROVIDER: S-EPMC54074 | biostudies-other | 1990 Jun
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 19900601 11
DNA damage generated by oxygen radicals includes base-free apurinic/apyrimidinic (AP) sites and strand breaks that bear deoxyribose fragments. The yeast Saccharomyces cerevisiae repairs such DNA lesions by using a single major enzyme. We have cloned the yeast structural gene (APN1) encoding this AP endonuclease/3'-repair diesterase by immunological screening of a yeast genomic DNA expression library in lambda gt11. Gene disruption experiments confirm that the Apn1 protein accounts for greater th ...[more]