Ontology highlight
ABSTRACT:
SUBMITTER: Sabatino DE
PROVIDER: S-EPMC2925478 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Sabatino Denise E DE Freguia Christian Furlan CF Toso Raffaella R Santos Andrey A Merricks Elizabeth P EP Kazazian Haig H HH Nichols Timothy C TC Camire Rodney M RM Arruda Valder R VR
Blood 20090921 20
Production of recombinant B-domain-deleted canine factor VIII (cFVIII-BDD) unexpectedly revealed superior protein yields with 3-fold increased specific activity relative to human FVIII-BDD (hFVIII-BDD). We also determined that activated cFVIII-BDD is more stable than activated hFVIII-BDD. Furthermore, cFVIII-BDD is efficient at inducing hemostasis in human plasma containing FVIII inhibitors. Infusion of cFVIII-BDD in hemophilia A dogs resulted in correction of the disease phenotype with a pharma ...[more]