Obovatol attenuates microglia-mediated neuroinflammation by modulating redox regulation.

ABSTRACT:

Background and purpose

Obovatol isolated from the medicinal herb Magnolia obovata exhibits a variety of biological activities. Here, the effect of obovatol and its mechanism of action on microglial activation, neuroinflammation and neurodegeneration were investigated.

Experimental approach

In microglial BV-2 cells stimulated with lipopolysaccharide (LPS), we measured nitric oxide (NO) and cytokine production, and activation of intracellular signalling pathways by reverse transcription-polymerase chain reaction and Western blots. Cell death was assayed in co-cultures of activated microglia (with bacterial LPS) and neurons and in LPS- induced neuroinflammation in mice in vivo.

Key results

Obovatol inhibited microglial NO production with an IC50 value of 10 mM. Obovatol

SUBMITTER: Ock J 

PROVIDER: S-EPMC2925488 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature