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A designer biomimetic vector with a chimeric architecture for targeted gene transfer.

ABSTRACT: Designer biomimetic vectors are genetically engineered biomacromolecules that are designed to mimic viral characteristics in order to overcome the cellular barriers associated with the targeted gene transfer. The vector in this study was genetically engineered to contain at precise locations: a) four tandem repeating units of N-terminal domain of histone H2A to condense DNA into stable nanosize particles suitable for cellular uptake, b) a model targeting motif to target HER2 and enhance internalization of nanoparticles, and c) a pH-responsive synthetic fusogenic peptide to disrupt endosome membranes and promote escape of the nanoparticles into the cytosol. The results demonstrate that a fully functional, multi-domain, designer vector can be engineered to target cells with high specificity, overcome the biological barriers associated with targeted gene transfer, and mediate efficient gene transfer.

SUBMITTER: Wang Y

PROVIDER: S-EPMC2925643 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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A designer biomimetic vector with a chimeric architecture for targeted gene transfer.

Wang Yuhua Y Mangipudi Sriramchandra Sastry SS Canine Brenda F BF Hatefi Arash A

Journal of controlled release : official journal of the Controlled Release Society 20090318 1

Designer biomimetic vectors are genetically engineered biomacromolecules that are designed to mimic viral characteristics in order to overcome the cellular barriers associated with the targeted gene transfer. The vector in this study was genetically engineered to contain at precise locations: a) four tandem repeating units of N-terminal domain of histone H2A to condense DNA into stable nanosize particles suitable for cellular uptake, b) a model targeting motif to target HER2 and enhance internal ...[more]

PMID: 19303038

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