Ontology highlight
ABSTRACT:
SUBMITTER: Rennoll SA
PROVIDER: S-EPMC4104357 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Rennoll Sherri A SA Scott Samantha A SA Yochum Gregory S GS
Biochemical and biophysical research communications 20140322 4
Designer TALEs (dTALEs) are chimeric transcription factors that can be engineered to regulate gene expression in mammalian cells. Whether dTALEs can block gene transcription downstream of signal transduction cascades, however, has yet to be fully explored. Here we tested whether dTALEs can be used to target genes whose expression is controlled by Wnt/β-catenin signaling. TALE DNA binding domains were engineered to recognize sequences adjacent to Wnt responsive enhancer elements (WREs) that contr ...[more]